 | CatalogNo.: K-2000D Description: TheCDP-MESynthase(IspD)EnzymaticAssayincludesallofthenecessarycomponentstoeffectivelymonitortheactivityoftheIspDenzyme.PyrophosphateisabyproductfromtheformationofCDP-MEwhichisthenconvertedtofreephosphateintheassaybypyrophosphatase.BymeasuringfreephosphatelevelstheusercaninfertheamountofMEPconvertedtoCDP-ME. | ||
Isoprenoidscompriseoneofthemostdiverseclassesofcompoundsfoundinnature.Withover50,000differentisoprenoidsidentifiedtodate,theyexhibitabroadrangeofstructuralcomplexityandareinvolvedinavarietyofBIOLOGicalfunctions.Electrontransport(quinones),stABIlizationofcellmembranes(hopanoidsandsterols),cellwallbiosynthesis(dolichols),signaltransduction(prenylatedproteins),photosynthesis(chlorophylls)andmodificationoftRNAsareamongtheprocessesthatinvolveisoprenoids.Isopentenyldiphosphate(IPP)anddimethylallyldiphosphate(DMAPP)aretheprecursorsforallisoprenoidcompoundsandtwounrelatedessentialpathwaysexistinnaturefortheirbiosynthesis.ThesetwoprecursorsareproducedbyeithertheMVAorMEPpathway.TheMVApathwayisfoundprimarilyineukaryotesandarchea,includinghumans,plantcytosolandsomeGram-positivebacteria,whiletheMEPpathwayisutilizedbymostbacteria,includingallGram-negatives,andplantchloroplasts.Duetothisnaturaldistribution,theMEPpathwayrepresentsapromisingtargetfordevelopmentofnovelantibacterialagentsandherbicides. StorageKitcanbestoredunopenedat-20oCforuptosixmonths.Allcomponentsandsolutionsshouldbeprotectedfromlight.RelatedProductsMEPPathwayReagentsIsoprenoids MEPPathwayinhibitors:Fosmidomycin(B-4201),FR900098(K-4202),5-Ketoclomazone(K-4101) CDP-MEPSynthesiskit(K-2000E) MEPPathwayEnzymes**COMINGSOON**:DXPSynthase,MEPSynthase,IspDEnzyme,IspEEnzyme,IspFEnzyme | |||
