 | CatalogNo.: E-20DXS Description: Inthefirststepofthemethylerythritolphosphate(MEP)pathwayforisoprenoidbiosynthesis,D-glyceraldehyde3-phosphate(GAP)andpyruvateundergoacondensationreactiontoproduce1-deoxy-D-xylulose5-phosphate(DXP)byDXPsynthase(DXS)inathiaminediphosphate(orthiaminepyrophosphate)dependentmanner. | ||
TheMEPpathwayisusedbymostbacteria,includingallGram-negativebacteria,forisoprenoidbiosynthesis.Isoprenoidscompriseoneofthemostdiverseclassesofcompoundsfoundinnature.Withover50,000differentisoprenoidsidentifiedtodtae,theyexhibitabroadrangeofstructuralcomplexityandareinvolvedinavarietyofBIOLOGicalfunctions[2].Electrontransport(quinones),stABIlizationofcellmembranes(hopanoidsandsterols),cellwallbiosynthesis(dolichols),signaltransductions(prenylatedproteins),photosynthesis(chlorophylls)andmodificationoftRNAsareamongtheprocessesthatinvolveisoprenoids[3].Isopentenyldiphosphate(IPP)anddimethylallyldisphosphate(DMAPP)aretheprecursorsforallisoprenoidcompoundsandtwounrelatedessentialpathwaysexistinnaturefortheirbiosynthesis.Thesetwoprecursorsareproducedbyeitherthemevalonate(MVA)orMEPpathway.TheMVApathwayisfoundprimarilyineukaryotes,includinghumans,plantcytosol,Archaea,andsomeGram-positivebacteria,whiletheMEPpathwayisutilizedbymostbacteriaandplantcholorplasts.Duetothisnaturaldistribution,theMEPpathwayrepresentsapromisingtargetfordevelopmentofnovelantibacterialagentsandherbicides[4]. StorageStoreproductfrozenbelow-20°C.Enzymewillbestableforatleast6monthsat-20°Casundilutedstock.FreezeinworkingaliquotstoavoidrepeatedthawingandfreezingReferences1)Bochar,D.A.;Freisen,J.A.;Stauffacher,C.V.andRodwell,V.W.(1999)inComprehensiveNaturalProductsChemistry,(Cane,D.Ed.)PergamonPress,Oxford,pp.15-44.2)Sacchettini,J.C.andPoulter,C.C.(1997)Science,277(5333),1788-9. 3)Testa,C.A.;Brown,M.J.(2003)CurrentPharmceuticalBiotechnology,4,248-259. RelatedProductsMEPPathwayIntermediatesandInhibitorsCDP-MESynthase(IspD)EnzymaticAssay(K-2000D) CDP-MEPSynthesisKit(K-2000E) | |||
