CatalogNo.: T-2000 Description: **PleaseContactEchelonBiosciencesforacustomquoteortodiscussprocessoftwopartscreening** | |||
TheWhole-CellMethylerythritolPhosphate(MEP)Pathway-SelectiveAntibacterialScreeningAssay,describedin[1],utilizesageneticallyengineeredstrainofSalmonellatyphimurium(CT31-7d)thatcanutilizeeithertheMEPorthemevalonate(MVA)pathwaysinordertoproduceisopentenyldiphosphate(IPP)anddimethylallyldiphosphate(DMAPP),thetwoessentialprecursorsforallisoprenoids.IntheabsenceofarABInoseandMVA,theMEPpathwayisutilizedforgrowth.WhensupplementedwitharabinoseandMVA,theengineeredMVApathwayisactivated.CompoundsinhibitingMEPpathwaygrowthwithnoeffectonMVApathwaygrowthareselectivefortheMEPpathway.ValidatedusingknownMEPpathwayinhibitorsandotherantibacterials,thescreeningplatformismoresensitivetoMEPpathwayinhibitorsthanwild-typeS.typhimurium.Antibacterialstargetingotherpathwaysdemonstratesimilarminimuminhibitoryconcentrations(MICs)forbothMEPandMVApathwaygrowth. |