ScientificBackground- Akt1orProteinKinaseBα(PKBα)isaserine/threoninekinasethatbelongstotheAktfamily.Akt1isactivatedincellsinresponsetodiversestimulisuchashormones,growthfactorsandextracellularmatrixcomponentsandisinvolvedinglucosemetabolism,transcription,survival,cellproliferation,angiogenesis,andcellmotility(1).ThePI3Kgeneratesphosphatidylinositol-3,4,5-trisphosphate(PIP3),alipidsecondmessengeressentialforthetranslocationofAkt1totheplasmamembranewhereitisphosphorylatedandactivatedbyphosphoinositide-dependentkinase-1(PDK-1)(2)andphosphoinositide-dependentkinase-2(possIBLyILK)(3).Akt1hasnumerouscellularsubstratesincludingproteins,whichpromotetheinhibitionofapoptosissuchastheForkheadtranscriptionfactorsandtheBcl-2familymemberBad(4).Inaddition,thecyclindependentkinaseinhibitorsaresubstratesofAktwhichwhenphosphorylatedrelinquishtheirinhibitoryinfluenceoncellcycleprogression.Aktalsome
Storage- Storeproductfrozenatorbelow-70°C.Stablefor1yearat-70°Casundilutedstock.Aliquottoavoidrepeatedthawingandfreezing.
References- 1.CofferPJ,JinJ,WoodgettJR.ProteinkinaseB(c-Akt):amultifunctionalmediatorofphosphatidylinositol3-kinaseactivation.BiochemJ.1998Oct1;335(Pt1):1-13.
2.AndersonKE,CoadwellJ,HawkinsPT.TranslocationofPDK-1totheplasmamembraneisimportantinallowingPDK-1toactivateproteinkinaseB.CurrBiol.1998Jun4;8(12):684-91.
3.PersadS,AttwellS,GrayV,MawjiN,DengJT,LeungD,YanJ,SangheraJ,DedharS.RegulationofproteinkinaseB/Akt-serine473phosphorylationbyintegrin-linkedkinase:criticalrolesforkinaseactivityandaminoacidsarginine211andserine343.JBiolChem.2001Jul20;276(29):27462-9.
4.SenP,MukherjeeS,RayD,RahaS.InvolvementoftheAkt/PKBsignalingpathwaywithdiseaseprocesses.MolCellBiochem.2003Nov;253(1-2):241-6.
5.NicholsonKM,AndersonNG.TheproteinkinaseB/Aktsignallingpathwayinhumanmalignancy.CellSignal.2002May;14(5):381-95.
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Viskase常用产品报价单产品名货号公司分类商城分类美金价Viskase/透析袋 MD25（3500）/25mm/MD25-3.5MD25-3.5透析透析1543.14
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