4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
联合碳化(授权代理)
主营:实验室透析袋
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Lipid_metabolism_study > echelon-inc/PKA cb, active/5ug/E-K033-5ug
商品详细echelon-inc/PKA cb, active/5ug/E-K033-5ug
echelon-inc/PKA cb, active/5ug/E-K033-5ug
echelon-inc/PKA cb, active/5ug/E-K033-5ug
商品编号: E-K033-5ug
品牌: 联合碳化
市场价: ¥3160.00
美元价: 1580.00
产地: 美国(厂家直采)
公司:
产品分类: 脂质代谢研究
公司分类: Lipid_metabolism_study
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Zoom
 
CatalogNo.: E-K033

Description: Recombinantfull-lengthhumanPKAcbcontainingN-terminalGSTtagwasexpressedbybaculovirusinSf9insectcells.ThegeneaccessionnumberisNM_002731.
Thismaterialissoldforresearchpurposesonly.
SpecificActivity
29umolphosphateincorporatedintoCREBtidesubstrateperminutepermgproteinat30°Cfor15minutesusingafinalconcentrationof50µMATP(0.83µCi/assay).

ScientificBackground

  • MostoftheeffectsofcAMParemediatedthroughthephosphorylationoftargetproteinsonserineorthreonineresiduesbythecAMP-dependentproteinkinase(AMPK).TheinactiveholoenzymeofAMPKisatetramercomposedoftworegulatoryandtwocatalyticsubunits.ThemammaliancatalyticsubunithasbeenshowntoconsistofthreePKAgeneproducts:C-alpha,C-beta,andC-gamma.TwoPKAisoformsexist,designatedtypesIandII,whichdifferintheirdimericregulatorysubunits,designatedRIandRII,respectively.FurThermore,thereareatleastfourdifferentregulatorysubunits:RI-alpha,RI-beta,RII-alpha,andRII-beta.ThecAMPcausesthedissociationoftheinactiveholoenzymeintoadimerofregulatorysubunitsboundtofourcAMPandtwofreemonomericcatalyticsubunits.ThecatalyticsubunitC-betaofPKA(PKAcb)isamemberoftheSer/ThrproteinkinasefamilyandisacatalyticsubunitC-betaofAMPK.Berubeetal.assignedthePKAcbtohumanchromosome1bySouthernblotanalysisofsomaticc

    Storage

    • Storeproductfrozenatorbelow-70°C.Stablefor1yearat-70°Casundilutedstock.Aliquottoavoidrepeatedthawingandfreezing.

    References

    • 1.Berube,D.;Simard,J.;Sandberg,M.;Grzeschik,K.-H.;Gagne,R.;Hansson,V.;Jahnsen,T.:AssignmentofthegeneencodingthecatalyticsubunitC(beta)ofcAMP-dependentproteinkinasetothep36bandonchromosome1.(Abstract)Cytogenet.CellGenet.58:1850only,1991.2.Simard,J.;Berube,D.;Sandberg,M.;Grzeschik,K.-H.;Gagne,R.;Hansson,V.;Jahnsen,T.:AssignmentofthegeneencodingthecatalyticsubunitC-betaofcAMP-dependentproteinkinasetothep36bandonchromosome1.Hum.Genet.88:653-657,1992.
    品牌介绍
    Viskase常用产品报价单产品名货号公司分类商城分类美金价Viskase/透析袋 MD25(3500)/25mm/MD25-3.5MD25-3.5透析透析1543.14