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当前位置: 首页 > 产品中心 > Lipid_metabolism_study > echelon-inc/PKC delta, active/20ug/E-K037-20ug
商品详细echelon-inc/PKC delta, active/20ug/E-K037-20ug
echelon-inc/PKC delta, active/20ug/E-K037-20ug
echelon-inc/PKC delta, active/20ug/E-K037-20ug
商品编号: E-K037-20ug
品牌: 联合碳化
市场价: ¥9480.00
美元价: 4503.00
产地: 美国(厂家直采)
公司:
产品分类: 脂质代谢研究
公司分类: Lipid_metabolism_study
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

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CatalogNo.: E-K037

Description: Recombinantfull-lengthhumanPKCdeltacontainingN-terminalGSTtagwasexpressedbybaculovirusinSf9insectcells.
ThegeneaccessionnumberisNM_006254.
Thismaterialissoldforresearchpurposesonly.
SpecificActivity
302nmolphosphateincorporatedintoCREBtidesubstrateperminutepermgproteinat30°Cfor15minutesusingafinalconcentrationof50µMATP(0.83µCi/assay).

ScientificBackground

  • ProteinkinaseCdelta(PKCdelta)isamemberoftheproteinkinaseC(PKC)familyofserine-threoninekinases.Itisa79kdproteinkinasethatshowsstrictdependenceonthepresenceofphospholipids,butshowsnoactivationbyCa2+(1).PhosphatidylinositolisthemostpotentactivatorofPKCdelta.Apparentkineticconstantsforsyntheticoligopeptides(MBP4-14,EGFRpeptideandepsilon-peptide)arequitedifferentbetweenPKCdeltaandotherPKCs.NorthernblotanalysisindicatedthatPKCdeltaiswidelydistributedinalmostallthetissuesandisamajorisoformofPKCexpressedinhemopoieticcells(2).PKCdeltaisinvolvedinfundamentalcellularfunctionsregulatedbydiacylglycerolsandmimickedbyphorbolesters.PKCdeltaispartiallyassociatedwiththeinsolublefractionincellsevenintheabsenceofphorbol12-myristate13-acetate(PMA).UponPMAstimulation,bothittranslocatetotheinsolublefractionofcellhomogenates(3).OverexpressionofPKC-deltainducessignificantchanges

    Storage

    • Storeproductfrozenatorbelow-70°C.Stablefor1yearat-70°Casundilutedstock.Aliquottoavoidrepeatedthawingandfreezing.

    References

    • 1.LeiberspergerH,GschwendtM,GernoldM,MarksF.Immunologicaldemonstrationofacalcium-unresponsiveproteinkinaseCofthedelta-typeindifferentspeciesandmurinetissues.Predominanceinepidermis.JBiolChem.1991Aug5;266(22):14778-84.2.MischakH,BodenteichA,KolchW,GoodnightJ,HoferF,MushinskiJF.MouseproteinkinaseC-delta,themajorisoformexpressedinmousehemopoieticcells:sequenceoftheCDNA,expressionpatterns,andcharacterizationoftheprotein.Biochemistry.1991Aug13;30(32):7925-31.3.MischakH,GoodnightJA,KolchW,Martiny-BaronG,SchaechtleC,KazanietzMG,BlumbergPM,PierceJH,MushinskiJF.OverexpressionofproteinkinaseC-deltaand-epsiloninNIH3T3cellsinducesoppositeeffectsongrowth,morphology,anchoragedependence,andtumOrigenicity.JBiolChem.1993Mar25;268(9):6090-6.
    品牌介绍
    Viskase常用产品报价单产品名货号公司分类商城分类美金价Viskase/透析袋 MD25(3500)/25mm/MD25-3.5MD25-3.5透析透析1543.14