 | |
CatalogNo.: K-1900
Description: TheS1PELISAisasensitiveandrobustmethodforthequantificationofS1PinBIOLOGicalsamples(96-wellformat).
SampleType:Serum/Plasma,TissueHomogenate,andCellculturelysate.
Speciesofsample:TheS1Pantibodyislikelynotspeciesspecific.TheS1PELISAhasbeentestedwithS1Pfromhuman,bovine,equine,caprine,andmouse.
AssayRange:2μM-0.0625μM
SampleVolume:25μLserumorplasmaforduplicatepoints.Fortissuehomogenateandcellculturesamples,pleaseseethesamplepreparationsectionofthetechnicaldatasheet. | |
ProductBackground:TheS1Pcoated96-wellmicrotiterisblockedtoreducenon-specificbinding.TheuserthenmixestheS1Pstandardcurveandsampleswiththeanti-S1PantibodybeforeaddingthemixturetotheblockedS1Pcoatedplate.Theanti-S1PantibodybindstotheS1PcoatedplateortheS1Pinthesample.Followinganincubationandplatewash,streptavidin-HRPisaddedtotheplateandbindstoallanti-S1Pantibodies(labeledwithbiotin)boundtotheplate.Afteranadditionalincubationandplatewash,TMBsubstrateisaddedtotheplateandthecolorometricreactionstoppedbytheadditionof1Nsulfuricacid.Theabsorbanceat450nmismeasuredandtheconcentrationofS1Pinthesamplesaredeterminedbycomparisontothestandardcurve.
Sphingosine1-Phosphate(S1P)iskeycomponentofthesphingolipidsignalingcascade.S1Pinitiatesaproliferative,pro-angiogenicandanti-apoptoticsequenceofeventscontributingtocancerprogression.Recently,scientificliteraturehassuggestedthatS1PisapotenttumOrigenicgrowthfactorthatislikelyreleasedfromtumorcellsandthatS1PmaybeanovelbioMarkerforearlystagecancerdetection.Sphingosinekinasehasalsobeenshowntobeup-regulatedinavarietyofcancertypes(S1Pisproducedviatheactivityofsphingosinekinasephosphorylatingsphingosine).
FeaturedinPublications:
1.Lai,W.Q.,A.W.Irwan,etal.(2008)."Anti-inflammatoryeffectsofsphingosinekinasemodulationininflammatoryarthritis."JImmunol181(11):8010-7.
2.Kirby,R.J.,Y.Jin,etal.(2009)."Dynamicregulationofsphingosine-1-phosphatehomeostasisduringdevelopmentofmousemetanephrickidney."AmJPhysiolRenalPhysiol296(3):F634-41.
3.Nincheri,P.,C.Bernacchioni,etal.(2010)."Sphingosinekinase-1/S1P1signallingaxisnegativelyregulatesmitogenicresponseelicitedbyPDGFinmousemyoblasts."CellSignal22(11):1688-99.
4.Roviezzo,F.,V.Brancaleone,etal.(2011)."Sphingosine-1-PhosphateModulatesVascularPermeABIlityandCellRecruitmentinAcuteInflammationInVivo."JournalofPharmacologyandExperimentalTherapeutics337(3):830-837.
Notetopurchaser:TheS1PAssaywasco-developedusingaproprietarymonoclonalantibodyfromLpathInc.(SanDiego,CA).Lpath,Inc.hasanumberofpatentapplicationspendingorissuedcoveringmaterial(s)incorporatedintothisassay.Theassay`sintendeduseisforthemeasurementofS1Pinbiologicalsamples.Anyotheruse,includingdrugdiscovery,requiresalicensefromLpath(www.lpath.com). |
