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当前位置: 首页 > 产品中心 > Lipid_metabolism_study > echelon inc/Autotaxin活性测定/1ea/K-4100-1ea
商品详细echelon inc/Autotaxin活性测定/1ea/K-4100-1ea
echelon inc/Autotaxin活性测定/1ea/K-4100-1ea
echelon inc/Autotaxin活性测定/1ea/K-4100-1ea
商品编号: K-4100-1ea
品牌: 联合碳化
市场价: ¥9000.00
美元价: 4275.00
产地: 美国(厂家直采)
公司:
产品分类: 脂质代谢研究
公司分类: Lipid_metabolism_study
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

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CatalogNo.: K-4100

Description: Echelon'sAutotaxinActivityAssaygivesaquantitativemeasurementofautotaxin(ATX)activityinBIOLOGicalsamplesbymeasuringchangesinfluorescenceovertime.

SampleVolume:400μL/sample(50μLperwell,8wellspersample)
SampleNumber:10samples(induplicate)/96wellassay
SampleType:Currentlythiskithasbeentestedforthequantificationofautotaxinactivityinserum,plasma,ovarianascites,andcellculturemedia.

ProductBackground:TheassaydevelopedbyEchelonBiosciencesInc.usesthefluorogenicautotaxinsubstrateFS-3.FS-3isanLPCanaloguethatisconjugatedwithbothafluorophoreandaquencher.Initsnativestatethequencherinterfereswiththefluorophore’sfluorescence.OnceautotaxincleavesFS-3,thefluorophorebecomesliberatedfromthequencher,resultinginincreasedfluorescence.
Thisassaygivesaquantitativemeasurementofautotaxinactivityinbiologicalsamplesbymeasuringchangesinfluorescenceovertime.Becausetheassayisnotendpointbased,ithastheadvantageofbeinglesssusceptIBLetoerrorarisingfrommistimingtheadditionofcomponentstodifferentsamplewells.Thefinalreadoutisinquantitativeunitsthatcorrectforinconsistenciesbetweendifferentusersequipment.

FeaturedinPublications:

1)Shelton,E.L.,C.L.Galindo,etal.(2013)."AutotaxinSignalingGovernsPhenotypicHeterogeneityinVisceralandParietalMesothelia."PLoSONE8(7):e69712.
2)Gaetano,C.G.,N.SamADI,etal.(2009)."Inhibitionofautotaxinproductionoractivityblockslysophosphatidylcholine-inducedmigrationofhumanbreastcancerandmelanomacells."MolecularCarcinogenesis48(9):801.
3)Braeuer,R.R.,M.Zigler,etal.(2012)."Galectin-3ContributestoMelanomaGrowthandMetastasisviaRegulationofNFAT1andAutotaxin."CancerResearch72(22):5757-5766.

RelatedProducts:
AutotaxinInhibitorScreeningKits:96-well(K-4200)and384-well(K-4200HTS)
AutotaxinSubstrate:FS-3(L-2000)
AutotaxinInhibitors:BrP-LPA(L-7416),HA130(B-0701),PF8380(B-0702),andThio-ccPA(L-7118)
AutotaxinEnzyme,active(E-4000)

NoticetoPurchaser:EchelonBiosciencesproductsaresoldforresearchanddevelopmentpurposesonlyandarenottobeincorporatedintoproductsforresalewithoutwrittenpermissionfromEchelonBiosciences.ThecompoundFS-3anditsuseinassayingforLysophospholipaseDactivityarecoveredbyEchelonBiosciencesInc.USPatent7,989,663.Thepurchaseofthisproductincludesalimited,non-transferableimmunityfromsuitundertheforegoingpatentclaimsforusingonlythisamountofproductforthepurchaser'sowninternalresearch.

ProductKeywords:ATX,LPA,FS-3,LPC,lysophospholipaseD

品牌介绍
Viskase常用产品报价单产品名货号公司分类商城分类美金价Viskase/透析袋 MD25(3500)/25mm/MD25-3.5MD25-3.5透析透析1543.14